Spectroscopic coherent Raman imaging (CRI) methods allow label-free, chemically specific imaging of materials and biological systems, and are opening up many exciting possibilities for understanding phenomena in these systems. I will briefly introduce broadband spectroscopic coherent anti-Stokes Raman scattering (BCARS) microscopy, discussing some of the key concepts that make this method practical. I will also present selected application examples from studies on C. elegans metabolism and human cytomegalovirus infection that highlight the utility of BCARS for discovering new biology through in-depth characterization of highly complex biological systems.

© 2019 The Author(s)

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