Abstract

We propose a single-shot 3D fluorescence microscope that achieves large field-of-view and good resolution across a wide axial range. This is implemented by inserting a diffuser into the Fourier plane of the objective and solving an inverse problem. We show experimental 3D results of a freely-moving C. elegans nematode at 25 fps.

© 2019 The Author(s)

PDF Article

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription