Abstract

Second-harmonic generation microscopy can provide estimation of some local molecule distribution properties. However, in order not to get erroneous conclusions, it is important to detect measurements with insufficient precision. Such a detection technique is developed considering an approximation of the ultimate precision provided by the Cramer–Rao bound. This method is characterized and a simple approximation of its detection and false alarm probabilities is developed.

© 2016 Optical Society of America

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