Abstract

The dual-color dynamic particle tracking approach that uses temporal focusing multiphoton fluorescence excitation and two-channel astigmatic imaging is utilized to track molecular trajectories in three dimensions to explore molecular interactions. Images of two fluorophores were obtained to extract their positions by optical sectioning excitation using a fast temporal focusing multiphoton excitation microscope (TFMPEM) and by the simultaneous collection of data in two channels. The presented pair of cylindrical lenses, which was used to adjust the astigmatism effect with the minimum shifting of the imaging plane, was more feasible and flexible than single cylindrical lens for aligning two separate detection channels in astigmatic imaging. The lateral and axial positioning resolutions were observed to be approximately 9-13 nm and 23-30 nm respectively, for the two fluorescence channels. The dynamic movement and binding behavior of clusters of GM-CSF receptors and JAK2 kinases in HeLa cells in the presence of GM-CSF ligands were observed. Therefore, the proposed dual-color tracking strategy is useful for the dynamic study of molecular interactions in living specimens with a fast frame rate, less photobleaching, better penetration depth, and minimum optical trapping force.

© 2015 Optical Society of America

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Corrections

25 August 2016: A correction was made to the author listing.


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References

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2014 (5)

2013 (1)

P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-throughput nonlinear optical microscopy,” Biophys. J. 105(12), 2641–2654 (2013).
[Crossref] [PubMed]

2012 (8)

I. Izeddin, M. El Beheiry, J. Andilla, D. Ciepielewski, X. Darzacq, and M. Dahan, “PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking,” Opt. Express 20(5), 4957–4967 (2012).
[Crossref] [PubMed]

I. Izeddin, M. El Beheiry, J. Andilla, D. Ciepielewski, X. Darzacq, and M. Dahan, “PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking,” Opt. Express 20(5), 4957–4967 (2012).
[Crossref] [PubMed]

J.-H. Spille, T. Kaminski, H.-P. Königshoven, and U. Kubitscheck, “Dynamic three-dimensional tracking of single fluorescent nanoparticles deep inside living tissue,” Opt. Express 20(18), 19697–19707 (2012).
[Crossref] [PubMed]

L. van de Laar, P. J. Coffer, and A. M. Woltman, “Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy,” Blood 119(15), 3383–3393 (2012).
[Crossref] [PubMed]

S. E. Broughton, T. R. Hercus, A. F. Lopez, and M. W. Parker, “Cytokine receptor activation at the cell surface,” Curr. Opin. Struct. Biol. 22(3), 350–359 (2012).
[Crossref] [PubMed]

S. E. Broughton, U. Dhagat, T. R. Hercus, T. L. Nero, M. A. Grimbaldeston, C. S. Bonder, A. F. Lopez, and M. W. Parker, “The GM-CSF/IL-3/IL-5 cytokine receptor family: from ligand recognition to initiation of signaling,” Immunol. Rev. 250(1), 277–302 (2012).
[Crossref] [PubMed]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
[Crossref] [PubMed]

P.-H. Chen, F.-C. Chien, S.-P. Lee, W.-E. Chan, I.-H. Lin, C.-S. Liu, F.-J. Lee, J.-S. Lai, P. Chen, H.-F. Yang-Yen, and J. J.-Y. Yen, “Identification of a novel function of the clathrin-coated structure at the plasma membrane in facilitating GM-CSF receptor-mediated activation of JAK2,” Cell Cycle 11(19), 3611–3626 (2012).
[Crossref] [PubMed]

2011 (3)

H. Dana and S. Shoham, “Numerical evaluation of temporal focusing characteristics in transparent and scattering media,” Opt. Express 19(6), 4937–4948 (2011).
[Crossref] [PubMed]

M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
[Crossref] [PubMed]

D. Entenberg, J. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[Crossref] [PubMed]

2010 (4)

I. Testa, C. A. Wurm, R. Medda, E. Rothermel, C. von Middendorf, J. Fölling, S. Jakobs, A. Schönle, S. W. Hell, and C. Eggeling, “Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength,” Biophys. J. 99(8), 2686–2694 (2010).
[Crossref] [PubMed]

A. Vaziri and C. V. Shank, “Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing,” Opt. Express 18(19), 19645–19655 (2010).
[Crossref] [PubMed]

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

D. Montiel and H. Yang, “Real-time three-dimensional single-particle tracking spectroscopy for complex systems,” Laser Photonics Rev. 4(3), 374–385 (2010).
[Crossref]

2008 (7)

W. Vainchenker, A. Dusa, and S. N. Constantinescu, “JAKs in pathology: Role of Janus kinases in hematopoietic malignancies and immunodeficiencies,” Semin. Cell Dev. Biol. 19(4), 385–393 (2008).
[Crossref] [PubMed]

G. Hansen, T. R. Hercus, B. J. McClure, F. C. Stomski, M. Dottore, J. Powell, H. Ramshaw, J. M. Woodcock, Y. Xu, M. Guthridge, W. J. McKinstry, A. F. Lopez, and M. W. Parker, “The structure of the GM-CSF receptor complex reveals a distinct mode of cytokine receptor activation,” Cell 134(3), 496–507 (2008).
[Crossref] [PubMed]

B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science 319(5864), 810–813 (2008).
[Crossref] [PubMed]

V. Nikolenko, B. O. Watson, R. Araya, A. Woodruff, D. S. Peterka, and R. Yuste, “SLM microscopy: scanless two-photon imaging and photostimulation with spatial light modulators,” Front. Neural Circuits 2(5), 1–14 (2008).
[PubMed]

M. E. Durst, G. Zhu, and C. Xu, “Simultaneous spatial and temporal focusing in nonlinear microscopy,” Opt. Commun. 281(7), 1796–1805 (2008).
[Crossref] [PubMed]

J. A. Hamilton, “Colony-stimulating factors in inflammation and autoimmunity,” Nat. Rev. Immunol. 8(7), 533–544 (2008).
[Crossref] [PubMed]

N. S. Makarov, M. Drobizhev, and A. Rebane, “Two-photon absorption standards in the 550-1600 nm excitation wavelength range,” Opt. Express 16(6), 4029–4047 (2008).
[Crossref] [PubMed]

2007 (1)

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90(5), 053902 (2007).
[Crossref]

2006 (1)

2005 (3)

V. Iyer, T. M. Hoogland, and P. Saggau, “Fast functional imaging of single neurons using random-access multiphoton (RAMP) microscopy,” J. Neurophysiol. 95(1), 535–545 (2005).
[Crossref] [PubMed]

D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
[Crossref] [PubMed]

V. Levi, Q. Ruan, and E. Gratton, “3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells,” Biophys. J. 88(4), 2919–2928 (2005).
[Crossref] [PubMed]

2003 (1)

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref] [PubMed]

2002 (1)

R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J. 82(5), 2775–2783 (2002).
[Crossref] [PubMed]

2000 (1)

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, “Two-photon excitation fluorescence microscopy,” Annu. Rev. Biomed. Eng. 2(1), 399–429 (2000).
[Crossref] [PubMed]

1997 (1)

M. J. Saxton, “Single-particle tracking: the distribution of diffusion coefficients,” Biophys. J. 72(4), 1744–1753 (1997).
[Crossref] [PubMed]

1994 (1)

H. P. Kao and A. S. Verkman, “Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position,” Biophys. J. 67(3), 1291–1300 (1994).
[Crossref] [PubMed]

1984 (1)

A. M. Mastro, M. A. Babich, W. D. Taylor, and A. D. Keith, “Diffusion of a small molecule in the cytoplasm of mammalian cells,” Proc. Natl. Acad. Sci. U.S.A. 81(11), 3414–3418 (1984).
[Crossref] [PubMed]

1983 (1)

J. R. Lepock, K.-H. Cheng, S. D. Campbell, and J. Kruuv, “Rotational diffusion of TEMPONE in the cytoplasm of Chinese hamster lung cells,” Biophys. J. 44(3), 405–412 (1983).
[Crossref] [PubMed]

Andilla, J.

Anselmi, F.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Araya, R.

V. Nikolenko, B. O. Watson, R. Araya, A. Woodruff, D. S. Peterka, and R. Yuste, “SLM microscopy: scanless two-photon imaging and photostimulation with spatial light modulators,” Front. Neural Circuits 2(5), 1–14 (2008).
[PubMed]

Babich, M. A.

A. M. Mastro, M. A. Babich, W. D. Taylor, and A. D. Keith, “Diffusion of a small molecule in the cytoplasm of mammalian cells,” Proc. Natl. Acad. Sci. U.S.A. 81(11), 3414–3418 (1984).
[Crossref] [PubMed]

Barretto, R. P. J.

Bates, M.

B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science 319(5864), 810–813 (2008).
[Crossref] [PubMed]

Bègue, A.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Berland, K. M.

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, “Two-photon excitation fluorescence microscopy,” Annu. Rev. Biomed. Eng. 2(1), 399–429 (2000).
[Crossref] [PubMed]

Bonder, C. S.

S. E. Broughton, U. Dhagat, T. R. Hercus, T. L. Nero, M. A. Grimbaldeston, C. S. Bonder, A. F. Lopez, and M. W. Parker, “The GM-CSF/IL-3/IL-5 cytokine receptor family: from ligand recognition to initiation of signaling,” Immunol. Rev. 250(1), 277–302 (2012).
[Crossref] [PubMed]

Broughton, S. E.

S. E. Broughton, U. Dhagat, T. R. Hercus, T. L. Nero, M. A. Grimbaldeston, C. S. Bonder, A. F. Lopez, and M. W. Parker, “The GM-CSF/IL-3/IL-5 cytokine receptor family: from ligand recognition to initiation of signaling,” Immunol. Rev. 250(1), 277–302 (2012).
[Crossref] [PubMed]

S. E. Broughton, T. R. Hercus, A. F. Lopez, and M. W. Parker, “Cytokine receptor activation at the cell surface,” Curr. Opin. Struct. Biol. 22(3), 350–359 (2012).
[Crossref] [PubMed]

Campbell, S. D.

J. R. Lepock, K.-H. Cheng, S. D. Campbell, and J. Kruuv, “Rotational diffusion of TEMPONE in the cytoplasm of Chinese hamster lung cells,” Biophys. J. 44(3), 405–412 (1983).
[Crossref] [PubMed]

Chan, W.-E.

P.-H. Chen, F.-C. Chien, S.-P. Lee, W.-E. Chan, I.-H. Lin, C.-S. Liu, F.-J. Lee, J.-S. Lai, P. Chen, H.-F. Yang-Yen, and J. J.-Y. Yen, “Identification of a novel function of the clathrin-coated structure at the plasma membrane in facilitating GM-CSF receptor-mediated activation of JAK2,” Cell Cycle 11(19), 3611–3626 (2012).
[Crossref] [PubMed]

Chang, C.-Y.

Chang, N.-S.

Chen, P.

P.-H. Chen, F.-C. Chien, S.-P. Lee, W.-E. Chan, I.-H. Lin, C.-S. Liu, F.-J. Lee, J.-S. Lai, P. Chen, H.-F. Yang-Yen, and J. J.-Y. Yen, “Identification of a novel function of the clathrin-coated structure at the plasma membrane in facilitating GM-CSF receptor-mediated activation of JAK2,” Cell Cycle 11(19), 3611–3626 (2012).
[Crossref] [PubMed]

Chen, P.-H.

P.-H. Chen, F.-C. Chien, S.-P. Lee, W.-E. Chan, I.-H. Lin, C.-S. Liu, F.-J. Lee, J.-S. Lai, P. Chen, H.-F. Yang-Yen, and J. J.-Y. Yen, “Identification of a novel function of the clathrin-coated structure at the plasma membrane in facilitating GM-CSF receptor-mediated activation of JAK2,” Cell Cycle 11(19), 3611–3626 (2012).
[Crossref] [PubMed]

Chen, S.-J.

Cheng, K.-H.

J. R. Lepock, K.-H. Cheng, S. D. Campbell, and J. Kruuv, “Rotational diffusion of TEMPONE in the cytoplasm of Chinese hamster lung cells,” Biophys. J. 44(3), 405–412 (1983).
[Crossref] [PubMed]

Cheng, L.-C.

Chien, F.-C.

Cho, K.-C.

Ciepielewski, D.

Cocker, E. D.

Coffer, P. J.

L. van de Laar, P. J. Coffer, and A. M. Woltman, “Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy,” Blood 119(15), 3383–3393 (2012).
[Crossref] [PubMed]

Condeelis, J.

D. Entenberg, J. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[Crossref] [PubMed]

Constantinescu, S. N.

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M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
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L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90(5), 053902 (2007).
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S. E. Broughton, U. Dhagat, T. R. Hercus, T. L. Nero, M. A. Grimbaldeston, C. S. Bonder, A. F. Lopez, and M. W. Parker, “The GM-CSF/IL-3/IL-5 cytokine receptor family: from ligand recognition to initiation of signaling,” Immunol. Rev. 250(1), 277–302 (2012).
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[Crossref] [PubMed]

G. Hansen, T. R. Hercus, B. J. McClure, F. C. Stomski, M. Dottore, J. Powell, H. Ramshaw, J. M. Woodcock, Y. Xu, M. Guthridge, W. J. McKinstry, A. F. Lopez, and M. W. Parker, “The structure of the GM-CSF receptor complex reveals a distinct mode of cytokine receptor activation,” Cell 134(3), 496–507 (2008).
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M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
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N. S. Makarov, M. Drobizhev, and A. Rebane, “Two-photon absorption standards in the 550-1600 nm excitation wavelength range,” Opt. Express 16(6), 4029–4047 (2008).
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D. Entenberg, J. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
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V. Levi, Q. Ruan, and E. Gratton, “3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells,” Biophys. J. 88(4), 2919–2928 (2005).
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V. Iyer, T. M. Hoogland, and P. Saggau, “Fast functional imaging of single neurons using random-access multiphoton (RAMP) microscopy,” J. Neurophysiol. 95(1), 535–545 (2005).
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Taylor, W. D.

A. M. Mastro, M. A. Babich, W. D. Taylor, and A. D. Keith, “Diffusion of a small molecule in the cytoplasm of mammalian cells,” Proc. Natl. Acad. Sci. U.S.A. 81(11), 3414–3418 (1984).
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M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
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W. Vainchenker, A. Dusa, and S. N. Constantinescu, “JAKs in pathology: Role of Janus kinases in hematopoietic malignancies and immunodeficiencies,” Semin. Cell Dev. Biol. 19(4), 385–393 (2008).
[Crossref] [PubMed]

van de Laar, L.

L. van de Laar, P. J. Coffer, and A. M. Woltman, “Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy,” Blood 119(15), 3383–3393 (2012).
[Crossref] [PubMed]

van de Linde, S.

Vaziri, A.

Verkhusha, V. V.

D. Entenberg, J. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
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Verkman, A. S.

H. P. Kao and A. S. Verkman, “Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position,” Biophys. J. 67(3), 1291–1300 (1994).
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von Middendorf, C.

I. Testa, C. A. Wurm, R. Medda, E. Rothermel, C. von Middendorf, J. Fölling, S. Jakobs, A. Schönle, S. W. Hell, and C. Eggeling, “Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength,” Biophys. J. 99(8), 2686–2694 (2010).
[Crossref] [PubMed]

Wang, W.

B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science 319(5864), 810–813 (2008).
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Watson, B. O.

V. Nikolenko, B. O. Watson, R. Araya, A. Woodruff, D. S. Peterka, and R. Yuste, “SLM microscopy: scanless two-photon imaging and photostimulation with spatial light modulators,” Front. Neural Circuits 2(5), 1–14 (2008).
[PubMed]

Webb, W. W.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
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R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J. 82(5), 2775–2783 (2002).
[Crossref] [PubMed]

Williams, R. M.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref] [PubMed]

Wolter, S.

Woltman, A. M.

L. van de Laar, P. J. Coffer, and A. M. Woltman, “Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy,” Blood 119(15), 3383–3393 (2012).
[Crossref] [PubMed]

Woodcock, J. M.

G. Hansen, T. R. Hercus, B. J. McClure, F. C. Stomski, M. Dottore, J. Powell, H. Ramshaw, J. M. Woodcock, Y. Xu, M. Guthridge, W. J. McKinstry, A. F. Lopez, and M. W. Parker, “The structure of the GM-CSF receptor complex reveals a distinct mode of cytokine receptor activation,” Cell 134(3), 496–507 (2008).
[Crossref] [PubMed]

Woodruff, A.

V. Nikolenko, B. O. Watson, R. Araya, A. Woodruff, D. S. Peterka, and R. Yuste, “SLM microscopy: scanless two-photon imaging and photostimulation with spatial light modulators,” Front. Neural Circuits 2(5), 1–14 (2008).
[PubMed]

Wurm, C. A.

I. Testa, C. A. Wurm, R. Medda, E. Rothermel, C. von Middendorf, J. Fölling, S. Jakobs, A. Schönle, S. W. Hell, and C. Eggeling, “Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength,” Biophys. J. 99(8), 2686–2694 (2010).
[Crossref] [PubMed]

Wyckoff, J.

D. Entenberg, J. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[Crossref] [PubMed]

Xu, C.

Xu, Y.

G. Hansen, T. R. Hercus, B. J. McClure, F. C. Stomski, M. Dottore, J. Powell, H. Ramshaw, J. M. Woodcock, Y. Xu, M. Guthridge, W. J. McKinstry, A. F. Lopez, and M. W. Parker, “The structure of the GM-CSF receptor complex reveals a distinct mode of cytokine receptor activation,” Cell 134(3), 496–507 (2008).
[Crossref] [PubMed]

Yang, H.

D. Montiel and H. Yang, “Real-time three-dimensional single-particle tracking spectroscopy for complex systems,” Laser Photonics Rev. 4(3), 374–385 (2010).
[Crossref]

Yang-Yen, H.-F.

P.-H. Chen, F.-C. Chien, S.-P. Lee, W.-E. Chan, I.-H. Lin, C.-S. Liu, F.-J. Lee, J.-S. Lai, P. Chen, H.-F. Yang-Yen, and J. J.-Y. Yen, “Identification of a novel function of the clathrin-coated structure at the plasma membrane in facilitating GM-CSF receptor-mediated activation of JAK2,” Cell Cycle 11(19), 3611–3626 (2012).
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P.-H. Chen, F.-C. Chien, S.-P. Lee, W.-E. Chan, I.-H. Lin, C.-S. Liu, F.-J. Lee, J.-S. Lai, P. Chen, H.-F. Yang-Yen, and J. J.-Y. Yen, “Identification of a novel function of the clathrin-coated structure at the plasma membrane in facilitating GM-CSF receptor-mediated activation of JAK2,” Cell Cycle 11(19), 3611–3626 (2012).
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Yen, W.-C.

Yew, E. Y. S.

P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-throughput nonlinear optical microscopy,” Biophys. J. 105(12), 2641–2654 (2013).
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Yih, J. N.

Yuste, R.

V. Nikolenko, B. O. Watson, R. Araya, A. Woodruff, D. S. Peterka, and R. Yuste, “SLM microscopy: scanless two-photon imaging and photostimulation with spatial light modulators,” Front. Neural Circuits 2(5), 1–14 (2008).
[PubMed]

Zhang, W.

Zhu, G.

M. E. Durst, G. Zhu, and C. Xu, “Simultaneous spatial and temporal focusing in nonlinear microscopy,” Opt. Commun. 281(7), 1796–1805 (2008).
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Zhuang, X.

B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science 319(5864), 810–813 (2008).
[Crossref] [PubMed]

Zipfel, W. R.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
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Annu. Rev. Biomed. Eng. (1)

P. T. C. So, C. Y. Dong, B. R. Masters, and K. M. Berland, “Two-photon excitation fluorescence microscopy,” Annu. Rev. Biomed. Eng. 2(1), 399–429 (2000).
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Appl. Phys. Lett. (1)

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90(5), 053902 (2007).
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Biomed. Opt. Express (1)

Biophys. J. (7)

I. Testa, C. A. Wurm, R. Medda, E. Rothermel, C. von Middendorf, J. Fölling, S. Jakobs, A. Schönle, S. W. Hell, and C. Eggeling, “Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength,” Biophys. J. 99(8), 2686–2694 (2010).
[Crossref] [PubMed]

P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-throughput nonlinear optical microscopy,” Biophys. J. 105(12), 2641–2654 (2013).
[Crossref] [PubMed]

R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J. 82(5), 2775–2783 (2002).
[Crossref] [PubMed]

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H. P. Kao and A. S. Verkman, “Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position,” Biophys. J. 67(3), 1291–1300 (1994).
[Crossref] [PubMed]

V. Levi, Q. Ruan, and E. Gratton, “3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells,” Biophys. J. 88(4), 2919–2928 (2005).
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M. J. Saxton, “Single-particle tracking: the distribution of diffusion coefficients,” Biophys. J. 72(4), 1744–1753 (1997).
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Blood (1)

L. van de Laar, P. J. Coffer, and A. M. Woltman, “Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy,” Blood 119(15), 3383–3393 (2012).
[Crossref] [PubMed]

Cell (1)

G. Hansen, T. R. Hercus, B. J. McClure, F. C. Stomski, M. Dottore, J. Powell, H. Ramshaw, J. M. Woodcock, Y. Xu, M. Guthridge, W. J. McKinstry, A. F. Lopez, and M. W. Parker, “The structure of the GM-CSF receptor complex reveals a distinct mode of cytokine receptor activation,” Cell 134(3), 496–507 (2008).
[Crossref] [PubMed]

Cell Cycle (1)

P.-H. Chen, F.-C. Chien, S.-P. Lee, W.-E. Chan, I.-H. Lin, C.-S. Liu, F.-J. Lee, J.-S. Lai, P. Chen, H.-F. Yang-Yen, and J. J.-Y. Yen, “Identification of a novel function of the clathrin-coated structure at the plasma membrane in facilitating GM-CSF receptor-mediated activation of JAK2,” Cell Cycle 11(19), 3611–3626 (2012).
[Crossref] [PubMed]

Curr. Opin. Struct. Biol. (1)

S. E. Broughton, T. R. Hercus, A. F. Lopez, and M. W. Parker, “Cytokine receptor activation at the cell surface,” Curr. Opin. Struct. Biol. 22(3), 350–359 (2012).
[Crossref] [PubMed]

Front. Neural Circuits (1)

V. Nikolenko, B. O. Watson, R. Araya, A. Woodruff, D. S. Peterka, and R. Yuste, “SLM microscopy: scanless two-photon imaging and photostimulation with spatial light modulators,” Front. Neural Circuits 2(5), 1–14 (2008).
[PubMed]

Immunol. Rev. (1)

S. E. Broughton, U. Dhagat, T. R. Hercus, T. L. Nero, M. A. Grimbaldeston, C. S. Bonder, A. F. Lopez, and M. W. Parker, “The GM-CSF/IL-3/IL-5 cytokine receptor family: from ligand recognition to initiation of signaling,” Immunol. Rev. 250(1), 277–302 (2012).
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J. Neurophysiol. (1)

V. Iyer, T. M. Hoogland, and P. Saggau, “Fast functional imaging of single neurons using random-access multiphoton (RAMP) microscopy,” J. Neurophysiol. 95(1), 535–545 (2005).
[Crossref] [PubMed]

Laser Photonics Rev. (1)

D. Montiel and H. Yang, “Real-time three-dimensional single-particle tracking spectroscopy for complex systems,” Laser Photonics Rev. 4(3), 374–385 (2010).
[Crossref]

Nat. Biotechnol. (1)

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref] [PubMed]

Nat. Methods (2)

M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
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E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Nat. Protoc. (1)

D. Entenberg, J. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
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Nat. Rev. Immunol. (1)

J. A. Hamilton, “Colony-stimulating factors in inflammation and autoimmunity,” Nat. Rev. Immunol. 8(7), 533–544 (2008).
[Crossref] [PubMed]

Opt. Commun. (1)

M. E. Durst, G. Zhu, and C. Xu, “Simultaneous spatial and temporal focusing in nonlinear microscopy,” Opt. Commun. 281(7), 1796–1805 (2008).
[Crossref] [PubMed]

Opt. Express (10)

H. Dana and S. Shoham, “Numerical evaluation of temporal focusing characteristics in transparent and scattering media,” Opt. Express 19(6), 4937–4948 (2011).
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I. Izeddin, M. El Beheiry, J. Andilla, D. Ciepielewski, X. Darzacq, and M. Dahan, “PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking,” Opt. Express 20(5), 4957–4967 (2012).
[Crossref] [PubMed]

J.-H. Spille, T. Kaminski, H.-P. Königshoven, and U. Kubitscheck, “Dynamic three-dimensional tracking of single fluorescent nanoparticles deep inside living tissue,” Opt. Express 20(18), 19697–19707 (2012).
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S. Proppert, S. Wolter, T. Holm, T. Klein, S. van de Linde, and M. Sauer, “Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging,” Opt. Express 22(9), 10304–10316 (2014).
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L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
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C.-H. Lien, C.-Y. Lin, S.-J. Chen, and F.-C. Chien, “Dynamic particle tracking via temporal focusing multiphoton microscopy with astigmatism imaging,” Opt. Express 22(22), 27290–27299 (2014).
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N. S. Makarov, M. Drobizhev, and A. Rebane, “Two-photon absorption standards in the 550-1600 nm excitation wavelength range,” Opt. Express 16(6), 4029–4047 (2008).
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A. Vaziri and C. V. Shank, “Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing,” Opt. Express 18(19), 19645–19655 (2010).
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D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
[Crossref] [PubMed]

I. Izeddin, M. El Beheiry, J. Andilla, D. Ciepielewski, X. Darzacq, and M. Dahan, “PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking,” Opt. Express 20(5), 4957–4967 (2012).
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Opt. Lett. (3)

Proc. Natl. Acad. Sci. U.S.A. (1)

A. M. Mastro, M. A. Babich, W. D. Taylor, and A. D. Keith, “Diffusion of a small molecule in the cytoplasm of mammalian cells,” Proc. Natl. Acad. Sci. U.S.A. 81(11), 3414–3418 (1984).
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Science (1)

B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science 319(5864), 810–813 (2008).
[Crossref] [PubMed]

Semin. Cell Dev. Biol. (1)

W. Vainchenker, A. Dusa, and S. N. Constantinescu, “JAKs in pathology: Role of Janus kinases in hematopoietic malignancies and immunodeficiencies,” Semin. Cell Dev. Biol. 19(4), 385–393 (2008).
[Crossref] [PubMed]

Other (2)

J. Bewersdorf, A. Egner, and S. W. Hell, “Multifocal multi-photon microscopy,” in Handbook of Biological Confocal Microscopy (Springer, 2006).

R. M. Mazo, Brownian Motion: Fluctuations, Dynamics, and Applications (Oxford University, 2009).

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Figures (7)

Fig. 1
Fig. 1 Optical configuration of dual-color dynamic tracking system with TFMPEM and astigmatism imaging
Fig. 2
Fig. 2 Simulation of astigmatic effect in light-collection optical path using one cylindrical lens. (a) Optical configuration of light-collection optical path. Cross-sectional patterns of aPSF at focal plane (b) with a cylindrical lens in various locations, and (c) with various angles of rotation of a cylindrical lens.
Fig. 3
Fig. 3 Simulation of astigmatic effect in light-collection optical path using two cylindrical lenses. (a) Optical configuration of imaging system. Cross-sectional patterns of aPSF at focal plane (b) with two cylindrical lenses at different angles, and (c) with the major axis of aPSF aligned in x or y direction.
Fig. 4
Fig. 4 Widths of images of multi-color fluorescent nanosphere in the x (blue points) and y (green points) directions, corresponding to different z positions, in (a) green and (b) red channels. Tops of Figs. 4 (a) and (b) show sectioned images at five positions with four sequential shifts of 750 nm in z direction. 3D position of a fixed fluorescent sphere as a function of time in (c) green and (d) red channels. Bottoms of Figs. 4 (c) and (d) show position histograms of the x, y, and z components.
Fig. 5
Fig. 5 (a) Merged image of free 1 μm green fluorescent sphere and 0.5 μm red fluorescent sphere; (b) 3D trajectory of both of fluorescent spheres in DI water with glycerol concentration of 30%; (c) MSDs of green and red fluorescent spheres.
Fig. 6
Fig. 6 Merged images of mRuby-tagged GMRβ (red) and clover-tagged JAK2 kinases (green) in living HeLa cells (a) with and (b) without GM-CSF treatment; (c) and (d) 3D trajectories of GMRβ and JAK2 kinase in white dashed square in Figs. 6(a) and 6(b), respectively.
Fig. 7
Fig. 7 (a) and (b) Fluorescent intensity-time plots on green and red fluorescent channels based on positions of GMRβ cluster in Figs. 6(c) and 6(d); (a) with and (b) without GM-CSF treatment. (c) Mean distance between clusters of GMRβ and JAK2 kinase in Figs. 6(c) and 6(d) with (blue line) and without (red line) GM-CSF treatment.

Equations (1)

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F W H M z k 1 + τ k 2 τ l + k 3 M N A 2

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