Abstract

This work addresses some key challenges in the fields of bio and nanophotonics by stimulated Raman microscopy. We present the design and the implementation of a femtosecond stimulated Raman scattering microscope, equipped with three femtosecond laser sources, which allows the coexistence of stimulated Raman gain (SRG) and stimulate Raman losses (SRL) detection modes in a single microscopy setup and to generate images of the same region in succession, without adding or removing components. In order to demonstrate the switching between the two detection modes, SRL and SRG images of polystyrene beads are acquired and the images quality are evaluated and compared.

© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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    [Crossref]
  4. L. Sirleto, M. A. Ferrara, G. Nicotra, C. Spinella, and I. Rendina, “Observation of stimulated Raman scattering in silicon nanocomposites,” Appl. Phys. Lett. 94(22), 221106 (2009).
    [Crossref]
  5. L. Sirleto, M. A. Ferrara, and A. Vergara, “Toward an ideal nanomaterial for on-chip Raman laser,” J. Nonlinear Opt. Phys. Mater. 26(03), 1750039 (2017).
    [Crossref]
  6. W. Min, C. W. Freudiger, S. Lu, and X. S. Xie, “Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy,” Annu. Rev. Phys. Chem. 62(1), 507–530 (2011).
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  7. D. Zhang, P. Wang, M. N. Slipchenko, and J.-X. Cheng, “Fast Vibrational Imaging of Single Cells and Tissues by Stimulated Raman Scattering Microscopy,” Acc. Chem. Res. 47(8), 2282–2290 (2014).
    [Crossref] [PubMed]
  8. J.-X. Cheng and X. S. Xie, “Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine,” Science 350(6264), aaa8870 (2015).
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  12. L. Wei, F. Hu, Z. Chen, Y. Shen, L. Zhang, and W. Min, “Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes,” Acc. Chem. Res. 49(8), 1494–1502 (2016).
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  15. L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
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    [Crossref] [PubMed]
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    [Crossref] [PubMed]
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    [Crossref] [PubMed]
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    [Crossref] [PubMed]
  24. A. D’Arco, N. Brancati, M. A. Ferrara, M. Indolfi, M. Frucci, and L. Sirleto, “Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques,” Biomed. Opt. Express 7(5), 1853–1864 (2016).
    [Crossref] [PubMed]
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    [Crossref]
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    [Crossref] [PubMed]
  27. C.-Y. Chung, J. Hsu, S. Mukamel, and E. O. Potma, “Controlling stimulated coherent spectroscopy and microscopy by a position-dependent phase,” Phys. Rev. A 87(3), 033833 (2013).
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    [Crossref]

2017 (5)

L. Sirleto, A. Vergara, and M. A. Ferrara, “Advances in stimulated Raman scattering in nanostructures,” Adv. Opt. Photonics 9(1), 169 (2017).
[Crossref]

L. Sirleto, M. A. Ferrara, and A. Vergara, “Toward an ideal nanomaterial for on-chip Raman laser,” J. Nonlinear Opt. Phys. Mater. 26(03), 1750039 (2017).
[Crossref]

C. Zhang, J. Li, L. Lan, and J. X. Cheng, “Quantification of Lipid Metabolism in Living Cells through the Dynamics of Lipid Droplets Measured by Stimulated Raman Scattering Imaging,” Anal. Chem. 89(8), 4502–4507 (2017).
[Crossref] [PubMed]

C. Zhang, K.-C. Huang, B. Rajwa, J. Li, S. Yang, H. Lin, C. Liao, G. Eakins, S. Kuang, V. Patsekin, J. P. Robinson, and J.-X. Cheng, “Stimulated Raman scattering flow cytometry for label-free single-particle analysis,” Optica 4(1), 103 (2017).
[Crossref]

A. D’Arco, M. A. Ferrara, M. Indolfi, V. Tufano, and L. Sirleto, “Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy,” J. Nonlinear Opt. Phys. Mater. 26(04), 1750052 (2017).
[Crossref]

2016 (3)

A. D’Arco, N. Brancati, M. A. Ferrara, M. Indolfi, M. Frucci, and L. Sirleto, “Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques,” Biomed. Opt. Express 7(5), 1853–1864 (2016).
[Crossref] [PubMed]

L. Wei, F. Hu, Z. Chen, Y. Shen, L. Zhang, and W. Min, “Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes,” Acc. Chem. Res. 49(8), 1494–1502 (2016).
[Crossref] [PubMed]

F. Hu, M. R. Lamprecht, L. Wei, B. Morrison, and W. Min, “Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering,” Sci. Rep. 6(1), 39660 (2016).
[Crossref] [PubMed]

2015 (5)

L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
[Crossref] [PubMed]

J.-X. Cheng and X. S. Xie, “Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine,” Science 350(6264), aaa8870 (2015).
[Crossref] [PubMed]

C. H. Camp and M. T. Cicerone, “Chemically sensitive bioimaging with coherent Raman scattering,” Nat. Photonics 9(5), 295–305 (2015).
[Crossref]

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

J. Li and J.-X. Cheng, “Direct Visualization of De novo Lipogenesis in Single Living Cells,” Sci. Rep. 4(1), 6807 (2015).
[Crossref] [PubMed]

2014 (4)

C.-R. Hu, D. Zhang, M. N. Slipchenko, J.-X. Cheng, and B. Hu, “Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy,” J. Biomed. Opt. 19(8), 086005 (2014).
[Crossref] [PubMed]

D. Zhang, P. Wang, M. N. Slipchenko, and J.-X. Cheng, “Fast Vibrational Imaging of Single Cells and Tissues by Stimulated Raman Scattering Microscopy,” Acc. Chem. Res. 47(8), 2282–2290 (2014).
[Crossref] [PubMed]

A. Alfonso-García, R. Mittal, E. S. Lee, and E. O. Potma, “Biological imaging with coherent Raman scattering microscopy: a tutorial,” J. Biomed. Opt. 19(7), 071407 (2014).
[Crossref] [PubMed]

L. Wei, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. C. Lin, M. C. Wang, and W. Min, “Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering,” Nat. Methods 11(4), 410–412 (2014).
[Crossref] [PubMed]

2013 (2)

A. Zumbusch, W. Langbein, and P. Borri, “Nonlinear vibrational microscopy applied to lipid biology,” Prog. Lipid Res. 52(4), 615–632 (2013).
[Crossref] [PubMed]

C.-Y. Chung, J. Hsu, S. Mukamel, and E. O. Potma, “Controlling stimulated coherent spectroscopy and microscopy by a position-dependent phase,” Phys. Rev. A 87(3), 033833 (2013).
[Crossref] [PubMed]

2012 (3)

K. I. Popov, A. F. Pegoraro, A. Stolow, and L. Ramunno, “Image formation in CARS and SRS: effect of an inhomogeneous nonresonant background medium,” Opt. Lett. 37(4), 473–475 (2012).
[Crossref] [PubMed]

L. Sirleto, M. A. Ferrara, T. Nikitin, S. Novikov, and L. Khriachtchev, “Giant Raman gain in silicon nanocrystals,” Nat. Commun. 3(1), 1220 (2012).
[Crossref] [PubMed]

W. Dou, D. Zhang, Y. Jung, J.-X. Cheng, and D. M. Umulis, “Label-Free Imaging of Lipid-Droplet Intracellular Motion in Early Drosophila Embryos Using Femtosecond-Stimulated Raman Loss Microscopy,” Biophys. J. 102(7), 1666–1675 (2012).
[Crossref] [PubMed]

2011 (2)

D. Zhang, M. N. Slipchenko, and J. X. Cheng, “Highly sensitive vibrational imaging by femtosecond pulse stimulated raman loss,” J. Phys. Chem. Lett. 2(11), 1248–1253 (2011).
[Crossref] [PubMed]

W. Min, C. W. Freudiger, S. Lu, and X. S. Xie, “Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy,” Annu. Rev. Phys. Chem. 62(1), 507–530 (2011).
[Crossref] [PubMed]

2009 (2)

Y. Ozeki, F. Dake, S. Kajiyama, K. Fukui, and K. Itoh, “Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy,” Opt. Express 17(5), 3651–3658 (2009).
[Crossref] [PubMed]

L. Sirleto, M. A. Ferrara, G. Nicotra, C. Spinella, and I. Rendina, “Observation of stimulated Raman scattering in silicon nanocomposites,” Appl. Phys. Lett. 94(22), 221106 (2009).
[Crossref]

2008 (2)

L. Sirleto, M. A. Ferrara, I. Rendina, S. N. Basu, J. Warga, R. Li, and L. Dal Negro, “Enhanced stimulated Raman scattering in silicon nanocrystals embedded in silicon-rich nitride/silicon superlattice structures,” Appl. Phys. Lett. 93(25), 251104 (2008).
[Crossref]

I. Rocha-Mendoza, W. Langbein, and P. Borri, “Coherent anti-Stokes Raman microspectroscopy using spectral focusing with glass dispersion,” Appl. Phys. Lett. 93(20), 201103 (2008).
[Crossref]

2004 (1)

T. Hellerer, A. M. K. Enejder, and A. Zumbusch, “Spectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses,” Appl. Phys. Lett. 85(1), 25–27 (2004).
[Crossref]

Alfonso-García, A.

A. Alfonso-García, R. Mittal, E. S. Lee, and E. O. Potma, “Biological imaging with coherent Raman scattering microscopy: a tutorial,” J. Biomed. Opt. 19(7), 071407 (2014).
[Crossref] [PubMed]

Barker, E. L.

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

Basu, S. N.

L. Sirleto, M. A. Ferrara, I. Rendina, S. N. Basu, J. Warga, R. Li, and L. Dal Negro, “Enhanced stimulated Raman scattering in silicon nanocrystals embedded in silicon-rich nitride/silicon superlattice structures,” Appl. Phys. Lett. 93(25), 251104 (2008).
[Crossref]

Borri, P.

A. Zumbusch, W. Langbein, and P. Borri, “Nonlinear vibrational microscopy applied to lipid biology,” Prog. Lipid Res. 52(4), 615–632 (2013).
[Crossref] [PubMed]

I. Rocha-Mendoza, W. Langbein, and P. Borri, “Coherent anti-Stokes Raman microspectroscopy using spectral focusing with glass dispersion,” Appl. Phys. Lett. 93(20), 201103 (2008).
[Crossref]

Brancati, N.

Buhman, K. K.

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

Camp, C. H.

C. H. Camp and M. T. Cicerone, “Chemically sensitive bioimaging with coherent Raman scattering,” Nat. Photonics 9(5), 295–305 (2015).
[Crossref]

Chen, Z.

L. Wei, F. Hu, Z. Chen, Y. Shen, L. Zhang, and W. Min, “Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes,” Acc. Chem. Res. 49(8), 1494–1502 (2016).
[Crossref] [PubMed]

L. Wei, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. C. Lin, M. C. Wang, and W. Min, “Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering,” Nat. Methods 11(4), 410–412 (2014).
[Crossref] [PubMed]

Cheng, J. X.

C. Zhang, J. Li, L. Lan, and J. X. Cheng, “Quantification of Lipid Metabolism in Living Cells through the Dynamics of Lipid Droplets Measured by Stimulated Raman Scattering Imaging,” Anal. Chem. 89(8), 4502–4507 (2017).
[Crossref] [PubMed]

D. Zhang, M. N. Slipchenko, and J. X. Cheng, “Highly sensitive vibrational imaging by femtosecond pulse stimulated raman loss,” J. Phys. Chem. Lett. 2(11), 1248–1253 (2011).
[Crossref] [PubMed]

Cheng, J.-X.

C. Zhang, K.-C. Huang, B. Rajwa, J. Li, S. Yang, H. Lin, C. Liao, G. Eakins, S. Kuang, V. Patsekin, J. P. Robinson, and J.-X. Cheng, “Stimulated Raman scattering flow cytometry for label-free single-particle analysis,” Optica 4(1), 103 (2017).
[Crossref]

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

J. Li and J.-X. Cheng, “Direct Visualization of De novo Lipogenesis in Single Living Cells,” Sci. Rep. 4(1), 6807 (2015).
[Crossref] [PubMed]

J.-X. Cheng and X. S. Xie, “Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine,” Science 350(6264), aaa8870 (2015).
[Crossref] [PubMed]

D. Zhang, P. Wang, M. N. Slipchenko, and J.-X. Cheng, “Fast Vibrational Imaging of Single Cells and Tissues by Stimulated Raman Scattering Microscopy,” Acc. Chem. Res. 47(8), 2282–2290 (2014).
[Crossref] [PubMed]

C.-R. Hu, D. Zhang, M. N. Slipchenko, J.-X. Cheng, and B. Hu, “Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy,” J. Biomed. Opt. 19(8), 086005 (2014).
[Crossref] [PubMed]

W. Dou, D. Zhang, Y. Jung, J.-X. Cheng, and D. M. Umulis, “Label-Free Imaging of Lipid-Droplet Intracellular Motion in Early Drosophila Embryos Using Femtosecond-Stimulated Raman Loss Microscopy,” Biophys. J. 102(7), 1666–1675 (2012).
[Crossref] [PubMed]

Chung, C.-Y.

C.-Y. Chung, J. Hsu, S. Mukamel, and E. O. Potma, “Controlling stimulated coherent spectroscopy and microscopy by a position-dependent phase,” Phys. Rev. A 87(3), 033833 (2013).
[Crossref] [PubMed]

Cicerone, M. T.

C. H. Camp and M. T. Cicerone, “Chemically sensitive bioimaging with coherent Raman scattering,” Nat. Photonics 9(5), 295–305 (2015).
[Crossref]

D’Arco, A.

A. D’Arco, M. A. Ferrara, M. Indolfi, V. Tufano, and L. Sirleto, “Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy,” J. Nonlinear Opt. Phys. Mater. 26(04), 1750052 (2017).
[Crossref]

A. D’Arco, N. Brancati, M. A. Ferrara, M. Indolfi, M. Frucci, and L. Sirleto, “Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques,” Biomed. Opt. Express 7(5), 1853–1864 (2016).
[Crossref] [PubMed]

Dai, M.

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

Dake, F.

Dal Negro, L.

L. Sirleto, M. A. Ferrara, I. Rendina, S. N. Basu, J. Warga, R. Li, and L. Dal Negro, “Enhanced stimulated Raman scattering in silicon nanocrystals embedded in silicon-rich nitride/silicon superlattice structures,” Appl. Phys. Lett. 93(25), 251104 (2008).
[Crossref]

Dou, W.

W. Dou, D. Zhang, Y. Jung, J.-X. Cheng, and D. M. Umulis, “Label-Free Imaging of Lipid-Droplet Intracellular Motion in Early Drosophila Embryos Using Femtosecond-Stimulated Raman Loss Microscopy,” Biophys. J. 102(7), 1666–1675 (2012).
[Crossref] [PubMed]

Eakins, G.

Enejder, A. M. K.

T. Hellerer, A. M. K. Enejder, and A. Zumbusch, “Spectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses,” Appl. Phys. Lett. 85(1), 25–27 (2004).
[Crossref]

Ferrara, M. A.

A. D’Arco, M. A. Ferrara, M. Indolfi, V. Tufano, and L. Sirleto, “Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy,” J. Nonlinear Opt. Phys. Mater. 26(04), 1750052 (2017).
[Crossref]

L. Sirleto, A. Vergara, and M. A. Ferrara, “Advances in stimulated Raman scattering in nanostructures,” Adv. Opt. Photonics 9(1), 169 (2017).
[Crossref]

L. Sirleto, M. A. Ferrara, and A. Vergara, “Toward an ideal nanomaterial for on-chip Raman laser,” J. Nonlinear Opt. Phys. Mater. 26(03), 1750039 (2017).
[Crossref]

A. D’Arco, N. Brancati, M. A. Ferrara, M. Indolfi, M. Frucci, and L. Sirleto, “Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques,” Biomed. Opt. Express 7(5), 1853–1864 (2016).
[Crossref] [PubMed]

L. Sirleto, M. A. Ferrara, T. Nikitin, S. Novikov, and L. Khriachtchev, “Giant Raman gain in silicon nanocrystals,” Nat. Commun. 3(1), 1220 (2012).
[Crossref] [PubMed]

L. Sirleto, M. A. Ferrara, G. Nicotra, C. Spinella, and I. Rendina, “Observation of stimulated Raman scattering in silicon nanocomposites,” Appl. Phys. Lett. 94(22), 221106 (2009).
[Crossref]

L. Sirleto, M. A. Ferrara, I. Rendina, S. N. Basu, J. Warga, R. Li, and L. Dal Negro, “Enhanced stimulated Raman scattering in silicon nanocrystals embedded in silicon-rich nitride/silicon superlattice structures,” Appl. Phys. Lett. 93(25), 251104 (2008).
[Crossref]

Freudiger, C. W.

W. Min, C. W. Freudiger, S. Lu, and X. S. Xie, “Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy,” Annu. Rev. Phys. Chem. 62(1), 507–530 (2011).
[Crossref] [PubMed]

Frucci, M.

Fukui, K.

Harrington, J. K.

L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
[Crossref] [PubMed]

Hellerer, T.

T. Hellerer, A. M. K. Enejder, and A. Zumbusch, “Spectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses,” Appl. Phys. Lett. 85(1), 25–27 (2004).
[Crossref]

Hsu, J.

C.-Y. Chung, J. Hsu, S. Mukamel, and E. O. Potma, “Controlling stimulated coherent spectroscopy and microscopy by a position-dependent phase,” Phys. Rev. A 87(3), 033833 (2013).
[Crossref] [PubMed]

Hu, B.

C.-R. Hu, D. Zhang, M. N. Slipchenko, J.-X. Cheng, and B. Hu, “Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy,” J. Biomed. Opt. 19(8), 086005 (2014).
[Crossref] [PubMed]

Hu, C.-R.

C.-R. Hu, D. Zhang, M. N. Slipchenko, J.-X. Cheng, and B. Hu, “Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy,” J. Biomed. Opt. 19(8), 086005 (2014).
[Crossref] [PubMed]

Hu, F.

F. Hu, M. R. Lamprecht, L. Wei, B. Morrison, and W. Min, “Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering,” Sci. Rep. 6(1), 39660 (2016).
[Crossref] [PubMed]

L. Wei, F. Hu, Z. Chen, Y. Shen, L. Zhang, and W. Min, “Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes,” Acc. Chem. Res. 49(8), 1494–1502 (2016).
[Crossref] [PubMed]

L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
[Crossref] [PubMed]

L. Wei, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. C. Lin, M. C. Wang, and W. Min, “Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering,” Nat. Methods 11(4), 410–412 (2014).
[Crossref] [PubMed]

Huang, K.-C.

Indolfi, M.

A. D’Arco, M. A. Ferrara, M. Indolfi, V. Tufano, and L. Sirleto, “Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy,” J. Nonlinear Opt. Phys. Mater. 26(04), 1750052 (2017).
[Crossref]

A. D’Arco, N. Brancati, M. A. Ferrara, M. Indolfi, M. Frucci, and L. Sirleto, “Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques,” Biomed. Opt. Express 7(5), 1853–1864 (2016).
[Crossref] [PubMed]

Itoh, K.

Jung, Y.

W. Dou, D. Zhang, Y. Jung, J.-X. Cheng, and D. M. Umulis, “Label-Free Imaging of Lipid-Droplet Intracellular Motion in Early Drosophila Embryos Using Femtosecond-Stimulated Raman Loss Microscopy,” Biophys. J. 102(7), 1666–1675 (2012).
[Crossref] [PubMed]

Kajiyama, S.

Khriachtchev, L.

L. Sirleto, M. A. Ferrara, T. Nikitin, S. Novikov, and L. Khriachtchev, “Giant Raman gain in silicon nanocrystals,” Nat. Commun. 3(1), 1220 (2012).
[Crossref] [PubMed]

Kuang, S.

Lamprecht, M. R.

F. Hu, M. R. Lamprecht, L. Wei, B. Morrison, and W. Min, “Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering,” Sci. Rep. 6(1), 39660 (2016).
[Crossref] [PubMed]

Lan, L.

C. Zhang, J. Li, L. Lan, and J. X. Cheng, “Quantification of Lipid Metabolism in Living Cells through the Dynamics of Lipid Droplets Measured by Stimulated Raman Scattering Imaging,” Anal. Chem. 89(8), 4502–4507 (2017).
[Crossref] [PubMed]

Langbein, W.

A. Zumbusch, W. Langbein, and P. Borri, “Nonlinear vibrational microscopy applied to lipid biology,” Prog. Lipid Res. 52(4), 615–632 (2013).
[Crossref] [PubMed]

I. Rocha-Mendoza, W. Langbein, and P. Borri, “Coherent anti-Stokes Raman microspectroscopy using spectral focusing with glass dispersion,” Appl. Phys. Lett. 93(20), 201103 (2008).
[Crossref]

Lee, E. S.

A. Alfonso-García, R. Mittal, E. S. Lee, and E. O. Potma, “Biological imaging with coherent Raman scattering microscopy: a tutorial,” J. Biomed. Opt. 19(7), 071407 (2014).
[Crossref] [PubMed]

Lee, H. J.

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

Li, J.

C. Zhang, J. Li, L. Lan, and J. X. Cheng, “Quantification of Lipid Metabolism in Living Cells through the Dynamics of Lipid Droplets Measured by Stimulated Raman Scattering Imaging,” Anal. Chem. 89(8), 4502–4507 (2017).
[Crossref] [PubMed]

C. Zhang, K.-C. Huang, B. Rajwa, J. Li, S. Yang, H. Lin, C. Liao, G. Eakins, S. Kuang, V. Patsekin, J. P. Robinson, and J.-X. Cheng, “Stimulated Raman scattering flow cytometry for label-free single-particle analysis,” Optica 4(1), 103 (2017).
[Crossref]

J. Li and J.-X. Cheng, “Direct Visualization of De novo Lipogenesis in Single Living Cells,” Sci. Rep. 4(1), 6807 (2015).
[Crossref] [PubMed]

Li, R.

L. Sirleto, M. A. Ferrara, I. Rendina, S. N. Basu, J. Warga, R. Li, and L. Dal Negro, “Enhanced stimulated Raman scattering in silicon nanocrystals embedded in silicon-rich nitride/silicon superlattice structures,” Appl. Phys. Lett. 93(25), 251104 (2008).
[Crossref]

Liao, C.

Lin, C. C.

L. Wei, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. C. Lin, M. C. Wang, and W. Min, “Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering,” Nat. Methods 11(4), 410–412 (2014).
[Crossref] [PubMed]

Lin, H.

Liu, B.

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

Lu, S.

W. Min, C. W. Freudiger, S. Lu, and X. S. Xie, “Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy,” Annu. Rev. Phys. Chem. 62(1), 507–530 (2011).
[Crossref] [PubMed]

Min, W.

L. Wei, F. Hu, Z. Chen, Y. Shen, L. Zhang, and W. Min, “Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes,” Acc. Chem. Res. 49(8), 1494–1502 (2016).
[Crossref] [PubMed]

F. Hu, M. R. Lamprecht, L. Wei, B. Morrison, and W. Min, “Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering,” Sci. Rep. 6(1), 39660 (2016).
[Crossref] [PubMed]

L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
[Crossref] [PubMed]

L. Wei, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. C. Lin, M. C. Wang, and W. Min, “Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering,” Nat. Methods 11(4), 410–412 (2014).
[Crossref] [PubMed]

W. Min, C. W. Freudiger, S. Lu, and X. S. Xie, “Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy,” Annu. Rev. Phys. Chem. 62(1), 507–530 (2011).
[Crossref] [PubMed]

Mittal, R.

A. Alfonso-García, R. Mittal, E. S. Lee, and E. O. Potma, “Biological imaging with coherent Raman scattering microscopy: a tutorial,” J. Biomed. Opt. 19(7), 071407 (2014).
[Crossref] [PubMed]

Morrison, B.

F. Hu, M. R. Lamprecht, L. Wei, B. Morrison, and W. Min, “Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering,” Sci. Rep. 6(1), 39660 (2016).
[Crossref] [PubMed]

Mukamel, S.

C.-Y. Chung, J. Hsu, S. Mukamel, and E. O. Potma, “Controlling stimulated coherent spectroscopy and microscopy by a position-dependent phase,” Phys. Rev. A 87(3), 033833 (2013).
[Crossref] [PubMed]

Nicotra, G.

L. Sirleto, M. A. Ferrara, G. Nicotra, C. Spinella, and I. Rendina, “Observation of stimulated Raman scattering in silicon nanocomposites,” Appl. Phys. Lett. 94(22), 221106 (2009).
[Crossref]

Nikitin, T.

L. Sirleto, M. A. Ferrara, T. Nikitin, S. Novikov, and L. Khriachtchev, “Giant Raman gain in silicon nanocrystals,” Nat. Commun. 3(1), 1220 (2012).
[Crossref] [PubMed]

Novikov, S.

L. Sirleto, M. A. Ferrara, T. Nikitin, S. Novikov, and L. Khriachtchev, “Giant Raman gain in silicon nanocrystals,” Nat. Commun. 3(1), 1220 (2012).
[Crossref] [PubMed]

Ozeki, Y.

Patsekin, V.

Pegoraro, A. F.

Popov, K. I.

Potma, E. O.

A. Alfonso-García, R. Mittal, E. S. Lee, and E. O. Potma, “Biological imaging with coherent Raman scattering microscopy: a tutorial,” J. Biomed. Opt. 19(7), 071407 (2014).
[Crossref] [PubMed]

C.-Y. Chung, J. Hsu, S. Mukamel, and E. O. Potma, “Controlling stimulated coherent spectroscopy and microscopy by a position-dependent phase,” Phys. Rev. A 87(3), 033833 (2013).
[Crossref] [PubMed]

Rajwa, B.

Ramunno, L.

Rendina, I.

L. Sirleto, M. A. Ferrara, G. Nicotra, C. Spinella, and I. Rendina, “Observation of stimulated Raman scattering in silicon nanocomposites,” Appl. Phys. Lett. 94(22), 221106 (2009).
[Crossref]

L. Sirleto, M. A. Ferrara, I. Rendina, S. N. Basu, J. Warga, R. Li, and L. Dal Negro, “Enhanced stimulated Raman scattering in silicon nanocrystals embedded in silicon-rich nitride/silicon superlattice structures,” Appl. Phys. Lett. 93(25), 251104 (2008).
[Crossref]

Robinson, J. P.

Rocha-Mendoza, I.

I. Rocha-Mendoza, W. Langbein, and P. Borri, “Coherent anti-Stokes Raman microspectroscopy using spectral focusing with glass dispersion,” Appl. Phys. Lett. 93(20), 201103 (2008).
[Crossref]

Shen, Y.

L. Wei, F. Hu, Z. Chen, Y. Shen, L. Zhang, and W. Min, “Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes,” Acc. Chem. Res. 49(8), 1494–1502 (2016).
[Crossref] [PubMed]

L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
[Crossref] [PubMed]

L. Wei, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. C. Lin, M. C. Wang, and W. Min, “Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering,” Nat. Methods 11(4), 410–412 (2014).
[Crossref] [PubMed]

Sirleto, L.

L. Sirleto, A. Vergara, and M. A. Ferrara, “Advances in stimulated Raman scattering in nanostructures,” Adv. Opt. Photonics 9(1), 169 (2017).
[Crossref]

L. Sirleto, M. A. Ferrara, and A. Vergara, “Toward an ideal nanomaterial for on-chip Raman laser,” J. Nonlinear Opt. Phys. Mater. 26(03), 1750039 (2017).
[Crossref]

A. D’Arco, M. A. Ferrara, M. Indolfi, V. Tufano, and L. Sirleto, “Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy,” J. Nonlinear Opt. Phys. Mater. 26(04), 1750052 (2017).
[Crossref]

A. D’Arco, N. Brancati, M. A. Ferrara, M. Indolfi, M. Frucci, and L. Sirleto, “Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques,” Biomed. Opt. Express 7(5), 1853–1864 (2016).
[Crossref] [PubMed]

L. Sirleto, M. A. Ferrara, T. Nikitin, S. Novikov, and L. Khriachtchev, “Giant Raman gain in silicon nanocrystals,” Nat. Commun. 3(1), 1220 (2012).
[Crossref] [PubMed]

L. Sirleto, M. A. Ferrara, G. Nicotra, C. Spinella, and I. Rendina, “Observation of stimulated Raman scattering in silicon nanocomposites,” Appl. Phys. Lett. 94(22), 221106 (2009).
[Crossref]

L. Sirleto, M. A. Ferrara, I. Rendina, S. N. Basu, J. Warga, R. Li, and L. Dal Negro, “Enhanced stimulated Raman scattering in silicon nanocrystals embedded in silicon-rich nitride/silicon superlattice structures,” Appl. Phys. Lett. 93(25), 251104 (2008).
[Crossref]

Slipchenko, L. V.

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

Slipchenko, M. N.

C.-R. Hu, D. Zhang, M. N. Slipchenko, J.-X. Cheng, and B. Hu, “Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy,” J. Biomed. Opt. 19(8), 086005 (2014).
[Crossref] [PubMed]

D. Zhang, P. Wang, M. N. Slipchenko, and J.-X. Cheng, “Fast Vibrational Imaging of Single Cells and Tissues by Stimulated Raman Scattering Microscopy,” Acc. Chem. Res. 47(8), 2282–2290 (2014).
[Crossref] [PubMed]

D. Zhang, M. N. Slipchenko, and J. X. Cheng, “Highly sensitive vibrational imaging by femtosecond pulse stimulated raman loss,” J. Phys. Chem. Lett. 2(11), 1248–1253 (2011).
[Crossref] [PubMed]

Spinella, C.

L. Sirleto, M. A. Ferrara, G. Nicotra, C. Spinella, and I. Rendina, “Observation of stimulated Raman scattering in silicon nanocomposites,” Appl. Phys. Lett. 94(22), 221106 (2009).
[Crossref]

Stolow, A.

Targoff, K. L.

L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
[Crossref] [PubMed]

Tufano, V.

A. D’Arco, M. A. Ferrara, M. Indolfi, V. Tufano, and L. Sirleto, “Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy,” J. Nonlinear Opt. Phys. Mater. 26(04), 1750052 (2017).
[Crossref]

Umulis, D. M.

W. Dou, D. Zhang, Y. Jung, J.-X. Cheng, and D. M. Umulis, “Label-Free Imaging of Lipid-Droplet Intracellular Motion in Early Drosophila Embryos Using Femtosecond-Stimulated Raman Loss Microscopy,” Biophys. J. 102(7), 1666–1675 (2012).
[Crossref] [PubMed]

Vergara, A.

L. Sirleto, A. Vergara, and M. A. Ferrara, “Advances in stimulated Raman scattering in nanostructures,” Adv. Opt. Photonics 9(1), 169 (2017).
[Crossref]

L. Sirleto, M. A. Ferrara, and A. Vergara, “Toward an ideal nanomaterial for on-chip Raman laser,” J. Nonlinear Opt. Phys. Mater. 26(03), 1750039 (2017).
[Crossref]

Wang, M. C.

L. Wei, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. C. Lin, M. C. Wang, and W. Min, “Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering,” Nat. Methods 11(4), 410–412 (2014).
[Crossref] [PubMed]

Wang, P.

D. Zhang, P. Wang, M. N. Slipchenko, and J.-X. Cheng, “Fast Vibrational Imaging of Single Cells and Tissues by Stimulated Raman Scattering Microscopy,” Acc. Chem. Res. 47(8), 2282–2290 (2014).
[Crossref] [PubMed]

Warga, J.

L. Sirleto, M. A. Ferrara, I. Rendina, S. N. Basu, J. Warga, R. Li, and L. Dal Negro, “Enhanced stimulated Raman scattering in silicon nanocrystals embedded in silicon-rich nitride/silicon superlattice structures,” Appl. Phys. Lett. 93(25), 251104 (2008).
[Crossref]

Wei, L.

F. Hu, M. R. Lamprecht, L. Wei, B. Morrison, and W. Min, “Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering,” Sci. Rep. 6(1), 39660 (2016).
[Crossref] [PubMed]

L. Wei, F. Hu, Z. Chen, Y. Shen, L. Zhang, and W. Min, “Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes,” Acc. Chem. Res. 49(8), 1494–1502 (2016).
[Crossref] [PubMed]

L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
[Crossref] [PubMed]

L. Wei, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. C. Lin, M. C. Wang, and W. Min, “Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering,” Nat. Methods 11(4), 410–412 (2014).
[Crossref] [PubMed]

Xie, X. S.

J.-X. Cheng and X. S. Xie, “Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine,” Science 350(6264), aaa8870 (2015).
[Crossref] [PubMed]

W. Min, C. W. Freudiger, S. Lu, and X. S. Xie, “Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy,” Annu. Rev. Phys. Chem. 62(1), 507–530 (2011).
[Crossref] [PubMed]

Xu, F.

L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
[Crossref] [PubMed]

Yang, S.

Yang, Y.

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

Yu, Y.

L. Wei, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. C. Lin, M. C. Wang, and W. Min, “Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering,” Nat. Methods 11(4), 410–412 (2014).
[Crossref] [PubMed]

Zhang, C.

C. Zhang, K.-C. Huang, B. Rajwa, J. Li, S. Yang, H. Lin, C. Liao, G. Eakins, S. Kuang, V. Patsekin, J. P. Robinson, and J.-X. Cheng, “Stimulated Raman scattering flow cytometry for label-free single-particle analysis,” Optica 4(1), 103 (2017).
[Crossref]

C. Zhang, J. Li, L. Lan, and J. X. Cheng, “Quantification of Lipid Metabolism in Living Cells through the Dynamics of Lipid Droplets Measured by Stimulated Raman Scattering Imaging,” Anal. Chem. 89(8), 4502–4507 (2017).
[Crossref] [PubMed]

Zhang, D.

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

C.-R. Hu, D. Zhang, M. N. Slipchenko, J.-X. Cheng, and B. Hu, “Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy,” J. Biomed. Opt. 19(8), 086005 (2014).
[Crossref] [PubMed]

D. Zhang, P. Wang, M. N. Slipchenko, and J.-X. Cheng, “Fast Vibrational Imaging of Single Cells and Tissues by Stimulated Raman Scattering Microscopy,” Acc. Chem. Res. 47(8), 2282–2290 (2014).
[Crossref] [PubMed]

W. Dou, D. Zhang, Y. Jung, J.-X. Cheng, and D. M. Umulis, “Label-Free Imaging of Lipid-Droplet Intracellular Motion in Early Drosophila Embryos Using Femtosecond-Stimulated Raman Loss Microscopy,” Biophys. J. 102(7), 1666–1675 (2012).
[Crossref] [PubMed]

D. Zhang, M. N. Slipchenko, and J. X. Cheng, “Highly sensitive vibrational imaging by femtosecond pulse stimulated raman loss,” J. Phys. Chem. Lett. 2(11), 1248–1253 (2011).
[Crossref] [PubMed]

Zhang, L.

L. Wei, F. Hu, Z. Chen, Y. Shen, L. Zhang, and W. Min, “Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes,” Acc. Chem. Res. 49(8), 1494–1502 (2016).
[Crossref] [PubMed]

Zhang, W.

H. J. Lee, W. Zhang, D. Zhang, Y. Yang, B. Liu, E. L. Barker, K. K. Buhman, L. V. Slipchenko, M. Dai, and J.-X. Cheng, “Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol,” Sci. Rep. 5(1), 7930 (2015).
[Crossref] [PubMed]

Zumbusch, A.

A. Zumbusch, W. Langbein, and P. Borri, “Nonlinear vibrational microscopy applied to lipid biology,” Prog. Lipid Res. 52(4), 615–632 (2013).
[Crossref] [PubMed]

T. Hellerer, A. M. K. Enejder, and A. Zumbusch, “Spectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses,” Appl. Phys. Lett. 85(1), 25–27 (2004).
[Crossref]

Acc. Chem. Res. (2)

D. Zhang, P. Wang, M. N. Slipchenko, and J.-X. Cheng, “Fast Vibrational Imaging of Single Cells and Tissues by Stimulated Raman Scattering Microscopy,” Acc. Chem. Res. 47(8), 2282–2290 (2014).
[Crossref] [PubMed]

L. Wei, F. Hu, Z. Chen, Y. Shen, L. Zhang, and W. Min, “Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes,” Acc. Chem. Res. 49(8), 1494–1502 (2016).
[Crossref] [PubMed]

ACS Chem. Biol. (1)

L. Wei, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff, and W. Min, “Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling,” ACS Chem. Biol. 10(3), 901–908 (2015).
[Crossref] [PubMed]

Adv. Opt. Photonics (1)

L. Sirleto, A. Vergara, and M. A. Ferrara, “Advances in stimulated Raman scattering in nanostructures,” Adv. Opt. Photonics 9(1), 169 (2017).
[Crossref]

Anal. Chem. (1)

C. Zhang, J. Li, L. Lan, and J. X. Cheng, “Quantification of Lipid Metabolism in Living Cells through the Dynamics of Lipid Droplets Measured by Stimulated Raman Scattering Imaging,” Anal. Chem. 89(8), 4502–4507 (2017).
[Crossref] [PubMed]

Annu. Rev. Phys. Chem. (1)

W. Min, C. W. Freudiger, S. Lu, and X. S. Xie, “Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy,” Annu. Rev. Phys. Chem. 62(1), 507–530 (2011).
[Crossref] [PubMed]

Appl. Phys. Lett. (4)

L. Sirleto, M. A. Ferrara, I. Rendina, S. N. Basu, J. Warga, R. Li, and L. Dal Negro, “Enhanced stimulated Raman scattering in silicon nanocrystals embedded in silicon-rich nitride/silicon superlattice structures,” Appl. Phys. Lett. 93(25), 251104 (2008).
[Crossref]

L. Sirleto, M. A. Ferrara, G. Nicotra, C. Spinella, and I. Rendina, “Observation of stimulated Raman scattering in silicon nanocomposites,” Appl. Phys. Lett. 94(22), 221106 (2009).
[Crossref]

T. Hellerer, A. M. K. Enejder, and A. Zumbusch, “Spectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses,” Appl. Phys. Lett. 85(1), 25–27 (2004).
[Crossref]

I. Rocha-Mendoza, W. Langbein, and P. Borri, “Coherent anti-Stokes Raman microspectroscopy using spectral focusing with glass dispersion,” Appl. Phys. Lett. 93(20), 201103 (2008).
[Crossref]

Biomed. Opt. Express (1)

Biophys. J. (1)

W. Dou, D. Zhang, Y. Jung, J.-X. Cheng, and D. M. Umulis, “Label-Free Imaging of Lipid-Droplet Intracellular Motion in Early Drosophila Embryos Using Femtosecond-Stimulated Raman Loss Microscopy,” Biophys. J. 102(7), 1666–1675 (2012).
[Crossref] [PubMed]

J. Biomed. Opt. (2)

A. Alfonso-García, R. Mittal, E. S. Lee, and E. O. Potma, “Biological imaging with coherent Raman scattering microscopy: a tutorial,” J. Biomed. Opt. 19(7), 071407 (2014).
[Crossref] [PubMed]

C.-R. Hu, D. Zhang, M. N. Slipchenko, J.-X. Cheng, and B. Hu, “Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy,” J. Biomed. Opt. 19(8), 086005 (2014).
[Crossref] [PubMed]

J. Nonlinear Opt. Phys. Mater. (2)

L. Sirleto, M. A. Ferrara, and A. Vergara, “Toward an ideal nanomaterial for on-chip Raman laser,” J. Nonlinear Opt. Phys. Mater. 26(03), 1750039 (2017).
[Crossref]

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Figures (5)

Fig. 1
Fig. 1 Schematic layout of the f-SRS microscope. Ti:Sa = Titanium-Sapphire laser, OPO = Optical Parametric Oscillator, SHG = Second Harmonic Generator, M1 – M6 = Mirrors, FM = Flip Mirror, DM1, DM2, DM3 = dichroic mirrors, DM4, DM5 = notch filters, EOM = Electro-Optic Modulator, FG = Function Generator, GM = Galvo Mirror, PD1, PD2 = Photodiodes, DAQ = Data acquisition system, PC = Personal Computer.
Fig. 2
Fig. 2 Raman shift for Ti:Sa-SHG laser sources combination as a function of Ti:Sa pumping wavelengths. The vertical red line represents the range of Raman shift obtained pumping OPO by the Ti:Sa wavelength used in our experiment (810 nm). The vertical black line represents the largest range of Raman shift, which is obtained pumping OPO by the Ti:Sa wavelength of 830 nm. The saffron and blue lines indicate minimum and maximum Raman shift, for Ti:Sa-SHG laser sources combination obtained pumping the OPO into the range [740-880] nm.
Fig. 3
Fig. 3 Generation system with combination of. Ti:Sa, OPO and SHG.
Fig. 4
Fig. 4 Images of polystyrene beads taken from f-SRS microscope at 3054 cm−1. A) SRL image, scale bar: 6 µm; B) SRG image, scale bar: 3.5 µm.
Fig. 5
Fig. 5 Schematic of the evaluation process.

Tables (2)

Tables Icon

Table 1 Combinations of the pump wavelengths injection leading to generation of the second harmonic wavelengths

Tables Icon

Table 2 The estimated SNR values provided by the Deconvolution Express function of the Huygens software v. 17.04 (Scientific Volume Imaging) and by our in-house software are reported for comparison.

Equations (1)

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SNR= Mean(object) SD(background)

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