Abstract

Resolution of a few tens of nanometers has been achieved in fluorescence microscopy with photoswitchable molecules. However, for thick samples, the background brought by the crosstalk of unwanted on-state molecules is nonnegligible. Now we present a background suppression method by using two axial standing waves generated by the interference of two activation beams with the same phases and two deactivation beams with the opposite phases. With spatially incoherent illumination, most activated molecules are located in a thin layer. The performance of such method is simulated with the known photoswitching characteristic of Cy5. With suitable parameters, the thickness of the layer can reach 39nm (FWHM).

© 2010 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Axial plane single-molecule super-resolution microscopy of whole cells

Sha An, Karl Ferdinand Ziegler, Peiyi Zhang, Yu Wang, Tim Kwok, Fan Xu, Cheng Bi, Sandro Matosevic, Peng Yin, Tongcang Li, and Fang Huang
Biomed. Opt. Express 11(1) 461-479 (2020)

Defocused imaging exploits supercritical-angle fluorescence emission for precise axial single molecule localization microscopy

Philipp Zelger, Lisa Bodner, Lukas Velas, Gerhard J. Schütz, and Alexander Jesacher
Biomed. Opt. Express 11(2) 775-790 (2020)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription